RECOMBINANT TECHNIQUES FOR PRODUCTION OF NOVEL NATRIURETIC AND VASODILATOR PEPTIDES

摘要

The invention is directed to peptides of the formula R1-Cys-Phe-Gly-Arg-Lys-Met-Asp-Arg- Ile-Ser-Ser-Ser-Gly-Leu-Gly-Cys-R2 wherein R1 is selected from the group consisting of: (H); Gly; Ser-Gly-; Gly-Ser-Gly-; Gln-Gly-Ser-Gly-; Val-Gln-Gly-Ser-Gly-; Met-Val-Gln-Gly-Ser-Gly-; Lys-Met-Val-Gln-Gly-Ser-Gly-; Pro-Lys-Met-Val-Gln-Gly-Ser-Gly-; Ser-Pro-Lys Met-Val-Gln-Gly-Ser-Gly-; and R3-Ser-Pro-Lys-Met-Val-Gln-Gly-Ser-Gly-; wherein R3 represents at least a portion of the native presequence of the human brain natriuretic peptide disclosed herein; and R2 is (OH), NH2, NH' or NR'R" wherein R' and R" are independently lower alkyl (1-4C) or R2 is selected from the group consisting of: Lys; Lys-Val; Lys-Val-Leu; Lys-Val-Leu-Arg; Lys-Val-Leu-Arg-Lys; Lys-Val-Leu-Arg-Lys-His; or the amides (NH2, NHR' or NR'R") thereof and to recombinant materials for its production. The peptides of the invention are useful in indications wherein natriuretic activity is required.