发明名称 |
RNA amplification method requiring only one manipulation step |
摘要 |
An RNA amplification method is particularly useful for diagnosing bacterial or viral infections or genetic disorders and for cell typing. The method includes the steps of denaturing a solution containing RNA, synthesizing a first cDNA strand from a suitable primer in the presence of reverse transcriptase, denaturing the heteroduplex formed, synthesizing a second cDNA strand from a second primer in the presence of DNA polymerase and then subjecting the cDNA formed to a sufficient number of amplification cycles. All the reactants and solvents are first placed in the same container to provide a single manipulation step that avoids the risk of contamination.
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申请公布号 |
US5654143(A) |
申请公布日期 |
1997.08.05 |
申请号 |
US19950412229 |
申请日期 |
1995.03.27 |
申请人 |
BIO MERIEUX |
发明人 |
MALLET, FRANÇOIS;ORIOL, GUY;MANDRAND, BERNARD |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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