发明名称 |
A RAPID METHOD TO DETECT DUPLEX FORMATION IN SEQUENCING BY HYBRIDIZATION METHODS |
摘要 |
A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.
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申请公布号 |
WO9727329(A1) |
申请公布日期 |
1997.07.31 |
申请号 |
WO1997US01219 |
申请日期 |
1997.01.24 |
申请人 |
THE UNIVERSITY OF CHICAGO |
发明人 |
MIRZABEKOV, ANDREI;YERSHOV, GENNADIY;ZASLAVSKY, ALEXANDER;GUSCHIN, DIMITRY;TIMOFEEV, EDWARD;FLORENTIEV, VLADIMER;KIRILLOV, EUGENE |
分类号 |
B01J19/00;C12Q1/68;C40B40/06;C40B60/14;(IPC1-7):C12Q1/68 |
主分类号 |
B01J19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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