发明名称 |
Gene coding human epidermal growth factor and process for preparing the same |
摘要 |
PCT No. PCT/KR94/00036 Sec. 371 Date Dec. 23, 1994 Sec. 102(e) Date Dec. 23, 1994 PCT Filed Apr. 25, 1994 PCT Pub. No. WO94/25592 PCT Pub. Date Nov. 10, 1994The present invention relates to a novel gene coding human epidermal growth factor("hEGF") and a process for preparing the same employing a recombinant expression vector therefor. The hEGF gene of the invention is designed to contain codons ubiquitous in E. coli and the following restriction sites: HpaI at the 5' terminal, PstI at the 3' terminal and Bpu1102I, NsiI, MluI, Eco47III and AflII at a regular manner within its internal sequence. The present invention also provides a process for preparing hEGF by employing a expression vector pTE105 for hEGF, which contains expression cassette comprising Omp A leader sequence, translation termination sequence and transcription termination sequence and hEGF gene; and, replication origin of pUC19, tetracycline-resistant marker and a par site for stabilization in E. coli. The hEGF is produced massively in E. coli transformed with the pTE105(KCCM 10027).
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申请公布号 |
US5652120(A) |
申请公布日期 |
1997.07.29 |
申请号 |
US19940360841 |
申请日期 |
1994.12.23 |
申请人 |
DAEWOONG PHARMACEUTICAL CO., LTD. |
发明人 |
PARK, SEUNG KOOK;LEE, KANG MOON;NHO, KYOO SEUNG;KOH, YEO WOOK;KWON, CHANG HYUK;CHUNG, JU YOUNG;JEE, YOUNG SU;YU, YOUNG HYO |
分类号 |
C12N15/09;C07K14/485;C12N1/21;C12N15/18;C12N15/70;C12P21/02;C12R1/19;(IPC1-7):C12N15/18 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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