Complementary DNA and toxins

摘要

This invention relates to new derivatized solid supports and compounds having the formula: wherein S may be a solid support; L may be a chemical bond or a suitable inorganic or organic linker; Z may be -SO2- or -S-S-; R may be -OH, an H-phosphonate, an alkane-phosphonate, a phosphotriester, a phosphite triester, a phosphite diester, a phosphorothioate, a phosphorodithioate, a phosphoroamidate, a phosphoroamidite, -OR1, -SR1, a nucleotide, N, which may be substituted or modified in its sugar, phosphate or base, or an oligonucleotide of the formula -(N)g -R2, wherein N is as defined above which may be the same or different; g is an integer from one to two hundred; R1 is a suitable protecting group; and R2 may be an H-phosphonate, an alkane-phosphonate, a phosphotriester, a phosphite triester, a phosphite diester, a phosphorothioate, a phosphorodithioate, a phosphoroamidate, a phosphoroamidite, -OH, -OR1, -SR1, or -O-P(OCH2CH2CN)-O-CH2CH2ZCH2CH2OR1. Furthermore, this invention provides methods for preparing 3'-phosphate oligonucleotides, 5'-phosphate oligonucleotides, (3',5')-diphosphate oligonucleotides, 3'-phosphate oligonucleotide conjugates, 5'-phosphate oligonucleotide conjugates, and (3',5')-diphosphate oligonucleotide conjugates.