发明名称 |
NOVEL METHODS FOR THE CHARACTERIZATION OF COMPOUNDS WHICH STIMULATE STF-1 EXPRESSION IN PANCREATIC ISLET CELLS |
摘要 |
The present invention provides two embodiments. One is a method of testing for compounds which may induce STF-1 transcription. Pancreatic islet cells which express the STF-1/lacZ fusion gene are isolated and the effect of various compounds on STF-1 expression is measured by adding the compound of interest to STF-1/lacZ expressing cells. LacZ activity in control and treated cells is then quantitated by a colorimetric assay. Using this method, a large number of compounds can be screened and STF-1 inducing compounds can be identified readily. The other is a method of using the STF-1 promoter to mark insulin producing pancreatic islet cells in vivo. In this regard, the green fluorescent protein (GFP) served as an indicator. The introduction of the STF-1-green fluorescent protein transgene into pigs allows for the efficient and rapid recovery of insulin producing cells from the pancreas.
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申请公布号 |
WO9726360(A1) |
申请公布日期 |
1997.07.24 |
申请号 |
WO1997US01033 |
申请日期 |
1997.01.22 |
申请人 |
RESEARCH DEVELOPMENT FOUNDATION |
发明人 |
MONTMINY, MARC, R.;SHARMA, SEEMA |
分类号 |
C12N15/09;A61K48/00;C07K14/47;C12N5/00;C12N5/10;C12N15/00;C12N15/63;C12N15/85;C12Q1/26;C12Q1/37;C12Q1/68;(IPC1-7):C12N15/63 |
主分类号 |
C12N15/09 |
代理机构 |
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