DIRECT PCR AMPLIFICATION TECHNIQUE OF NUCLEIC ACID USING WHOLE BLOOD AS TEMPLATE

摘要

PROBLEM TO BE SOLVED: To provide a process which can readily amplify a target DNA sequence from untreated blood by a PCR amplification technique. SOLUTION: Fresh, and untreated blood or a preserved blood for transfusion is used as a template, a primer of a nucleotide complementary to a target DNA sequence, 4 kinds of deoxyribonucleotide triphosphates and a heat-resistant DNA polymerase, for example, Tth DNA polymerase orΔTth DNA polymerase are allowed to act on each other in a reaction solution containing 0.05-0.15% of a surfactent, for example, Triton-X100, heating and cooling are repeated to amplify the target DNA sequence in the blood. Or this technique is utilized to detect a specific nucleic acid in the blood.