| 摘要 |
The present invention relates to novel sequences encoding a novel bacteriocin, the uses thereof, as well as the use of sequences for homologous and heterologous protein expression and secretion purposes. The present invention relates more particularly to a polynucleic acid comprising: (a) at least 30 contiguous nucleotides of a sequence selected from the polynucleic acid sequence as shown in Figure 2 (SEQ ID NO: 19), (b) a polynucleic acid sequence which hybridizes to the polynucleic acid as defined in (a), or (c) a polynucleic acid with a sequence that is degenerated to a polynucleic acid as defined in (a) or (b) as a result of the genetic code. The polynucleic acid sequence represented as SEQ ID NO: 19 in Figure 2 comprises five operons (see Figure 3). The first two are composed of three open reading frames: two "bacteriocin-like" genes, followed by an "immunity gene". The third operon is composed of two open reading frames, the first is coding for an ATP-dependent transporter protein, the second is coding for an accessory protein. The fourth operon is composed of two open reading frames, one "bacteriocin-like" gene followed by an "immunity gene". The fifht operon is composed of three open reading frames: two "bacteriocin-like" genes, followed by an "immunity gene". Each operon is preced by a promoter. |
