发明名称 |
A METHOD AND USE OF POLYPEPTIDE IN SPERM-EGG BINDING TO ENHANCE OR DECREASE FERTILITY |
摘要 |
A purified polypeptide which provides for initial binding of sperm to oocyte investments and has an active amino acid sequence of SEQ ID NO:12 (Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn-Gln-Arg-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu-Val-Asp-Leu-Ala-Arg-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Arg-Pro-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn-Glu-Asp-Val-Cys); or SEQ ID NO:13 (Cys-Glu-Ser-Leu-Gln-Lys-His-Leu-Ala-Glu-Leu-Asn-His-Gln-Lys-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu-Leu-Asp-Met-Thr-Glu-Val-Val-Ala-Pro-Phe-Met-Ala-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Gly-Pro-Arg-Ser-Lys-Pro-Gln-Pro-Lys-Asp-Asn-Gly-Asp-Val-Cys); or the shorter but biologically active SEQ ID NO:1 and SEQ ID NO:9 (Tyr-Pro-Gln-Asp-Arg-X-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn, where X is Thr or Pro). The polypeptide is useful in improving sperm-egg binding by fresh sperm, restoring sperm-egg binding following a cryopreservation cycle, enhancing fertilizing potential, and producing antibodies to determine fertilizing potential of sperm, determining sperm-binding sites on egg investments, and contraception.
|
申请公布号 |
WO9725620(A1) |
申请公布日期 |
1997.07.17 |
申请号 |
WO1997US00105 |
申请日期 |
1997.01.06 |
申请人 |
THE PENN STATE RESEARCH FOUNDATION |
发明人 |
HAMMERSTEDT, ROY, H.;CRAMER, PALMER, G.;BARBATO, GUY, F. |
分类号 |
G01N33/50;A61K38/00;A61P15/00;A61P15/08;C07K7/08;C07K14/47;C07K14/705;G01N33/53;(IPC1-7):G01N33/53;A61K38/10;A61K38/17;C07K7/00;C07K14/00;C07K16/00 |
主分类号 |
G01N33/50 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|