发明名称 |
VECTORS AND PROCESS FOR PRODUCING HIGH PURITY 6,12-DIDEOXYERYTHROMYCIN A BY FERMENTATION |
摘要 |
A process for producing high purity 6,12-dideoxyerythromycin A using recombinant DNA technology is disclosed. The erythromycin producing strain, Saccharopolyspora erythraea, lacking the erythromycin C-12 and C-6 hydroxylases produces a mixture of 6,12-dideoxyerythromycin A and the precursor molecule, 6-deoxyerythromycin D. To achieve conversion of the precursor to the final product, a second copy of eryG is inserted into a non-essential region of the Sac. erythraea chromosome resulting in high purity 6,12-dideoxyerythromycin A. |
申请公布号 |
EP0783584(A1) |
申请公布日期 |
1997.07.16 |
申请号 |
EP19960928078 |
申请日期 |
1996.08.02 |
申请人 |
ABBOTT LABORATORIES |
发明人 |
STASSI, DIANE, L.;MAINE, GREGORY, T.;POST, DAVID, A.;SATTER, MARK |
分类号 |
C12N15/09;C12N5/10;C12N9/06;C12N9/10;C12N15/76;C12N15/90;C12P19/62 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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