Microbiological best method and reagents

摘要

A method for detecting the presence and/or mount of microorganisms is described by adding adenosine diphosphate (ADP) to a sample suspected of containing microorganisms and/or their intracellular material, determining the amount of adenosine triphosphate (ATP) generated by adenylate kinase present, for example using a bioluminescent assay involving luciferase and luciferin, and relating the results to the presence and/or amount of microorganism. Test kits and apparatus for use in the method are also described.