发明名称 |
Transposon-containing DNA cloning vector and uses thereof |
摘要 |
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.
|
申请公布号 |
US5645991(A) |
申请公布日期 |
1997.07.08 |
申请号 |
US19950403582 |
申请日期 |
1995.03.14 |
申请人 |
UNIV. OF CONNECTICUT;WASHINGTON UNIVERSITY |
发明人 |
BERG, CLAIRE M.;BERG, DOUGLAS E.;WANG, GAN |
分类号 |
C12N15/10;C12N15/85;(IPC1-7):C12N15/00 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|