摘要 |
PROBLEM TO BE SOLVED: To culture efficiently the subject microalgae which can fix CO2 with light and convert it into fuel, etc., without inhibiting multiplication by contamination with sundry germs by multiplying a microalga belonging to green algae in an open system while adding a disinfectant intermittently to the culture medium. SOLUTION: This method for efficiently culturing a microalga belonging to green algae, which has ability to fix CO2 by light energy and converts it into useful substances such as fuel comprises culturing microalgae (e.g. Botryococcus braunii CCAP807/1, etc.) belonging to such as Botryococcus, Chlorella, or Haematococcus, which belongs to green algae, while adding intermittently a disinfectant such as hypochlorous acid, hypochlorite, hydrogen peroxide and ozone to the culture medium intermittently each in a quantity of 0.01-200ppm so as to attain an effective quantity of the disinfectant in an open system under conditions of light illuminance of 10-50μE/m<2> .s and aeration of 0.05-5vvm air containing CO2 in concentration of 0.03-30% at culturing temperature of 10-40 deg.C for about 10 days. |