| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new yeast variant comprising Saccharomyces cerevisiae, whose multiplication is suppressed by an immunosuppressive agent FK506 of a specific concentration, and which is used for determining effects of immunosuppressive agents or screening the agents. SOLUTION: This new yeast variant highly sensitive to immunosuppressive agents comprises SU3 strain (FERM P-15362) of Saccharomyces-cerevisiae whose multiplication is suppressed by 0.1μg/ml of an immunosuppressive agent FK505, and useful as a reagent for determining effects of immunosuppressive agents or screening the agents. This yeast variant is obtained by using DV3T-A strain which is a malfunctioned variant of tonoplast, H<+> -ATPase, of Saccharomyces- cerevisiae as a parent strain, culturing the parent strain on a medium that contains ethylmethanesulfonic acid(EMS) as a mutagen to mutate the parent strain, screening the living germs on a culture medium containing the immunosuppressive agent FK506 in concentration of 0.1μg/l, and selecting the multiplication- suppressed strain. |
