Automatic focusing of biomedical specimens apparatus

摘要

A method for automatically focusing on a slide includes the steps of locating a coverslip, acquiring images from predetermined focal depths in the slide, and starting at an initial focal depth proximate the surface of the coverslip. A set of predetermined characteristics within each of the images are measured to generate at least one image measurement for each of the plurality of images. A focus measure is computed for each of the images, where each focus measure is a function of at least one image measurement. A best focus location is determined relative to a focal depth where an acquired image has a highest focus measure.