| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new DNA coding for Ascorbate oxidase(AOX) having also a specific amino acid sequence derived from a microorganism belonging to the genus Acremonium and having a specific amino acid sequence and useful for production of AOX for increasing accuracy of enzymatic reaction system by removing ascorbic acid. SOLUTION: This new DNA codes for ascorbate oxidase(AOX) polypeptide composed of an amino acid sequence comprising 1-551 of amino acid sequence represented by the formula and is useful in generic engineering production of AOX utilized for increasing accuracy of an enzymatic reaction system. The DNA is obtained by culturing Acremonium.chrysogenum ATCC 1155.pcs MAO1 (FERM-P-15329) in a culture medium, extracting DNA by ordinary method from a microbial cell collected by centrifugal separation, preparing a gene library using the DNA, screening the library with a probe and recovering DNA by a positive clone. |
