| 摘要 |
A DNA template consisting of multiple tandem repetitions of an oligonucleotide unit is produced by endless copying of a circular oligonucleotide comprising at least one copy of said oligonucleotide unit by means of a nucleic acid polymerase, which is capable of strand displacement and is substantially without 5'-3' exonuclease activity, in the presence of the necessary nucleoside triphosphates and, if necessary, a primer capable of binding to some portion of the oligonucleotide. This reaction can be used in a process for the amplification of a particular DNA sequence wherein said DNA sequence is either circularized or inserted into a circular oligonucleotide, and the resulting circular DNA is used as a template for an endless copying process. |
