Process for preparation of human glicentin

摘要

Disclosed is a process for the preparation of native human glicentin, which comprises producing by gene technology a fused protein composed of human glicentin having a peptide moiety connected to the N-terminus of human glicentin and removing the connected peptide moiety from the fused protein, the improvement in which a purification of the fused protein and human glicentin is carried out by utilizing a reversible covalent bond of a cystein residue with the stationary phase of a chromatography.