| 摘要 |
Disclosed are a method and apparatus for the precise positioning of cells into highly organized tissue structures, which are suitable for use in the treatment of burn patients. Specific placement of cells onto substrata is obtained by the computer-controlled direct placement of cell suspension matrix material, which includes cells suspended by an extracellular matrix material, including cell adhesion proteins, collagens, gelatin (i.e., degraded collagen), and cyanoacrylates or other cellular glues. A fluorescence activated cell sorter is used to provide a stream or droplet stream of the biological material. Cells may also be positioned by means of computer-controlled placement of cell adhesion materials such as fibronectin to a substratum which is suitable for maintaining cell growth, followed by allowing cells to attach to those areas of the substratum where the cell adhesion material has been placed. These apparati may even be further modified and improved by providing a controlled biological environment in the area of the substratum, thus providing an integral unit for the production of organized tissues.
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