| 摘要 |
PROBLEM TO BE SOLVED: To detect an optimal exciting wavelength and fluorescent wavelength correctly in a short time by scanning the fluorescent wavelength from the upper limit value to the lower limit value while shifting a specified wavelength interval of exciting light at a time thereby determining an accumulated fluorescent spectral data. SOLUTION: A selected 14 wavelengthλEXn of exciting light is shifted by a specified 24 wavelength intervalΔλEX of exciting light at a time from a lower limit valueλEX1 to an upper limit valueλEXn within a predetermined 22 shift range. A selected 18 fluorescent wavelengthλEMn is then scanned from the wavelengthλEXn of exciting light added with a specified 24 fluorescent differential wavelengthΔλ1 to the wavelengthλEXn added with a specified 24 fluorescent differential wavelengthΔλ2 . Fluorescent intensity of unit fluorescent spectral data thus obtained is then accumulated while being matched to that of an accumulated spectral data in a memory 34. Subsequently, a wavelengthλEX0 of exciting light having maximum fluorescent intensity is detected 44 from an exciting spectral data as an optimal exciting wavelength. Furthermore, a wavelengthλEM0 having maximum fluorescent intensity is detected 52 from the fluorescent spectral data of optimal exciting wavelengthλEX0 as an optimal fluorescent wavelength.
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