DNA construct and in vitro test for detecting tumor promoters by means of said DNA construct

摘要

PCT No. PCT/DE91/00652 Sec. 371 Date Jul. 25, 1994 Sec. 102(e) Date Jul. 25, 1994 PCT Filed Aug. 16, 1991 PCT Pub. No. WO93/04179 PCT Pub. Date Mar. 4, 1993The invention relates to a DNA construct and to a method of detecting tumor promoters by employing this DNA construct. It is an object of the invention to make available an easily implemented quantitative detection method for tumor promoters. This is accomplished by a DNA construct including at least one reporter gene and a control region for the activation of a particularly strongly induced gene of the Epstein-Barr virus (EBV), and by the action of the substance to be examined on EBV-containing cells that contain the DNA construct, whereupon the resulting quantity of reporter gene product is quantitatively determined.