发明名称 |
THERMOLABILE URACIL-DNA-GLYCOSYLASE, PROCESS FOR ITS PREPARATION AND USE FOR REMOVING URACIL FROM DNA |
摘要 |
The invention concerns a thermolabile enzyme having uracil-DNA-glycosylase activity and characterized in particular by a high degree of purity, short half-life times and a content of less than 2 % contaminating foreign activities. The invention further concerns a process for preparing this enzyme and its use for removing the base uracil from DNA, in particular uracil-containing PCR products. The enzyme can be obtained from Gram-positive microorganisms such as, for example, Arthrobacter or Micrococcus. |
申请公布号 |
WO9720922(A1) |
申请公布日期 |
1997.06.12 |
申请号 |
WO1996EP05398 |
申请日期 |
1996.12.04 |
申请人 |
BOEHRINGER MANNHEIM GMBH;SOBEK, HARALD;SCHMIDT, MANFRED;FREY, BRUNO;KALUZA, KLAUS |
发明人 |
SOBEK, HARALD;SCHMIDT, MANFRED;FREY, BRUNO;KALUZA, KLAUS |
分类号 |
C12N15/09;C12N9/24;C12N15/10;C12P19/34;C12Q1/68;C12R1/06;C12R1/265 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|