METHOD OF PREPARING CHITOSAN-GLUCANE COMPLEX

摘要

FIELD: biotechnology. SUBSTANCE: invention proposes method of preparing chitosan-glucane complex at content of glucane and chitosan from 25 to 60% and deacetylation degree from 15 to 70%. Biomass of Aspergillus niger is treated with 2 N hydrochloric acid, then - with a mixture of reagents (detergent, weak alkali, ethanol) at 96 C for 35-45 min. Deacetylation is carried out with 20-25% alkali for 1-2 h at 100-110 C. Then the end product is washed off with alcohols to remove alkali at decreasing concentration and with water. EFFECT: accelerated and universal method.