发明名称 |
METHODS FOR GENERATING POLYNUCLEOTIDES HAVING DESIRED CHARACTERISTICS BY ITERATIVE SELECTION AND RECOMBINATION |
摘要 |
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
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申请公布号 |
CA2239099(A1) |
申请公布日期 |
1997.06.05 |
申请号 |
CA19962239099 |
申请日期 |
1996.12.02 |
申请人 |
AFFYMAX TECHNOLOGIES N.V. |
发明人 |
CRAMERI, ANDREAS;STEMMER, WILLEM P. C. |
分类号 |
C12N15/09;C07K14/435;C07K14/46;C07K14/545;C07K16/00;C12N9/38;C12N9/86;C12N15/10;C12N15/52;C12N15/64;C12Q1/68;C40B40/02;H01L29/772;H01L29/78;H01L29/808;H01L39/22;(IPC1-7):C12N15/12 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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