发明名称 Method for purification and transfer to separation/detection systems of DNA sequencing samples and plates used therefor
摘要 Disclosed are: a part for assembling reaction vessels comprising a base plate with through-holes passing said plate in the direction of thickness; a method for preparation of reaction vessels containing a reaction solution characterized in that the part is immersed in a reaction solution to charge the through-holes with the reaction solution and then one of apertures of each hole of the above through-holes is sealed with a membrane to complete said reaction vessels; and a plate comprises a base plate with through-holes passing said plate in the direction of thickness and a membrane for sealing one of apertures of each hole of said through-holes. The plate is used for purification of DNA sequencing samples using a pressure difference or an electrical potential, and transferring DNA sequencing samples to a separation/detection system. By the means or the method of the invention, reaction solutions can be easily charged into a large number of wells in a short time, unreacted labeling substances and the like can be easily removed in a short time from a plurality of DNA sequencing samples, and a large number of DNA sequencing samples can be easily purified and/or transferred to electrophoresis capillaries in a short time. <IMAGE>
申请公布号 EP0776700(A1) 申请公布日期 1997.06.04
申请号 EP19960119652 申请日期 1996.12.07
申请人 THE INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH ( RIKEN) 发明人 HAYASHIZAKI, YOSHIHIDE
分类号 B01L3/00;C40B60/14;G01N27/447;(IPC1-7):B01L3/00 主分类号 B01L3/00
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