发明名称 |
Design and synthesis of bispecific DNA-antibody conjugates |
摘要 |
The invention relates to bis-protein-DNA conjugates. A protein having an antigen specific binding activity is covalently linked to each end of a derivatized DNA molecule. The bis-protein-DNA conjugates can be used for immunoassays and measuring distances between proteins at up to 3.4 ANGSTROM resolution. The invention also relates to methods of synthesizing these bis-protein-DNA conjugates. Synthesis of the conjugates entails derivatizing the 5' or 3' end of a DNA oligonucleotide and covalently linking that DNA to a protein. The DNA can be indirectly conjugated to an antibody or Fab' fragment, using a avidin/streptavidin-biotin linkage. The conjugates of the invention can be used in immunoassays and PCR assays.
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申请公布号 |
US5635602(A) |
申请公布日期 |
1997.06.03 |
申请号 |
US19930107186 |
申请日期 |
1993.08.13 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
CANTOR, CHARLES R.;CHUCK, ROY S.;TSE, DORIS B. |
分类号 |
C07K16/28;C07K16/46;(IPC1-7):C07K17/00;C07K19/00 |
主分类号 |
C07K16/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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