| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject compound useful as a preventive, a therapeutic agent, etc., for insulin-independent diabetic, by culturing a bacterium belonging to the genus Agrobacterium in a liquid medium containing myo-inositol and collecting a product accumulated in a culture solution. SOLUTION: A bacterium belonging to the genus Agrobacterium (e.g. Agrobacterium tumefaciens IAM1037 strain) is inoculated into a liquid medium containing myo-inositol and subjected to shaking culture at 27 deg.C for 3 days, a stereoisomer of inositol is formed and accumulated in a culture solution. The culture solution is centrifuged, a supernatant liquid is collected and the cultured supernatant liquid is successively subjected to a strongly acidic ion exchange resin, a strongly basic ion exchange resin, a high-performance liquid chromatography, etc., and fractioned to give the objective stereoisomer of inositol such as D-chiro-inositol useful for preventing and treating insulin-independent diabetic, L-chiro-inositol having information exchange action in vivo or L-scyllo- inositol for diagnosing a brain stem disease, etc. |
