NOVEL SYNTHETIC SUBSTRATE FOR ACTIVITY ASSAY HAVING CHROMOPHORE OR FLUOROCHROMOPHORE ACTIVE AGAINST HEPATITIS C VIRUS NS3 PROTEASE

摘要

A synthetic substrate which contains a specific amino acid sequence, has a fluorochromophore or chromophore covalently bonded to the C-terminus, and carries at least one amino acid inhibiting the aminopeptidase digestion on the N-terminal side of the above sequence. The use of this substrate makes it possible to efficiently assay the activity of an NS3 protease. By effecting the assay in the presence of NS4A, the detection sensitivity can be further elevated.