发明名称 |
Verwendung des Lectins Sambucus nigra zur Quantifizierung der endständigen Sialinsäurereste des Human-Transferrin-Moleküls mittels einer vollimmunoenzymatischen Methode (EIA) |
摘要 |
The object of the present invention is a process for detecting end-position sialic acid groups of human transferrin using the sandwich principle. The process is characterised in that the sample fluid containing the human transferrin is incubated with a first receptor capable of bonding specifically with human transferrin, the complex thus formed is separated from the sample fluid, incubated with a second receptor capable of binding specifically to end-position sialic acid groups of human transferrin, the second receptor being bound or capable of binding to a marker, and the complex of first receptor, human transferrin and second receptor is determined by the marker. In one embodiment, the claimed process facilitates determination of sialic acid-deficient human transferrin in body fluids.
|
申请公布号 |
DE19543569(A1) |
申请公布日期 |
1997.05.28 |
申请号 |
DE1995143569 |
申请日期 |
1995.11.22 |
申请人 |
LO, ATOU, 70565 STUTTGART, DE;LO, INSA, 70565 STUTTGART, DE |
发明人 |
LO, ATOU, 70565 STUTTGART, DE |
分类号 |
G01N33/68;(IPC1-7):G01N33/53;C12Q1/28 |
主分类号 |
G01N33/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|