Method for measuring specific gene expression: transcriptional activity per gene dose

摘要

A method of detecting the level of transcriptionally-related gene expression from a population of cells includes the steps of isolating mRNA and DNA from a population of cells, quantifying the amount of a specific subpopulation of target mRNA transcribed from the target DNA and normalizing the amount of quantified target mRNA to target DNA for measuring transcriptional activity of the target DNA; a measure of gene expression per gene dosage.