| 摘要 |
Methods, substances and compositions derived from leeches, bacteria associated with leeches, or combinations thereof are provided to prevent retroviral infections of host cells. More particularly, the invention relates to the inhibition of HIV replication. Thus the invention provides methods and substances for treating and/or preventing HIV infection and/or AIDS. The compounds or mixtures are obtainable by solvent extraction techniques and separation steps and were tested in the following manner. Two syncytiumforming HIV isolates, HIVAms55 and HIVAms37 were inoculated in PBMC and monocytes. During 14 days HIV induced cytopathic effects (CPE) (syncytium formation) and the virus production as determined by a p24 capture ELISA was monitored, after addition of leech head extracts. The result of these tests with the partly purified HIV inhibiting substance or substances is as follows: 3 fractions inhibited both syncytium formation and p24 antigen by 100 % at a concentration of: 0.067 .mu.M, 0.5 .mu.M and 0.17 .mu.M respectively in both HIV isolates. A fourth fraction underwent dilution series and inhibited both CPE and p-24 antigen 100 % from 0.5 .mu.M upwards in both HIV isolates.
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