Transgenic mouse expressing DNA sequences encoding the human poliovirus receptor

摘要

The subject invention provides an isolated nucleic acid molecule which comprises nucleic acid encoding a polypeptide which has the biological activity of a receptor for poliovirus and in addition, provides a purified polypeptide which has this activity. The subject invention also provides a method for inducing the production of a polypeptides which includes the use of expression vectors in a host vector system. Therapeutic compositions comprising purified polypeptides which have the biological activity of a receptor for poliovirus are also provided for as are methods of treating and preventing human poliovirus infection. Further, the subject invention provides a method of producing a transgenic animal expressing human poliovirus receptors. The subject invention provides a method of testing the efficacy of a poliovirus receptors vaccine which comprises administering the vaccine to a transgenic animal described above and determining whether the resulting transgenic animal is protected from infection by human picornavirus. Lastly, a method of testing the virulence of a picornavirus vaccine is provided.