USE OF O-.BETA.-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS FOR THE EVALUATION OF INTESTINAL LACTASE, AND PRODUCTION PROCESS

摘要

There is disclosed the use of O-.beta.-D-galactopyranosyl-D- xyloses, particularly 2-O-.beta.-D-galactopyranosyl-D-xylose, and 3-O-.beta.-D-galactopyranosyl--D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. There is also disclosed a process for producing O-.beta.-D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of .beta.-D- galactopyranoside in the presence of an enzyme .beta.- galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37.degree.C; deactivating the .beta.-galactosidase by heating at 100.degree.C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/alcohol.