METHOD FOR EXPRESSION CLONING OF GENE ENCODING LIGAND-BOUND PROTEIN

摘要

An efficient method for cloning a DNA which encodes a ligand-bound protein. The method comprises incubating on a plate a host into which a DNA inserted into an expression vector has been introduced; bringing a membrane into contact with the plate to thereby transfer the protein thus expressed from the plate onto the membrane; peeling off the membrane from the plate; adding a labeled ligand to the membrane so as to bind the ligand to the protein; detecting the label bound to the protein; and then isolating the DNA from a clone on the plate corresponding to the positive site on the membrane.