| 摘要 |
<p>New intercalating asymmetric cyanine dyes are provided in which the benzothiazole portion of the cyanine dye has been modified to produce dyes with improved properties for labelling nucleic acids, such as longer wavelengths and improved fluorescence enhancement when bound to DNA or RNA. More specifically, the dyes are cyclized fluorescent cyanine dyes for non-covalently labelling nucleic acids. Methods are described for detecting nucleic acids in a sample by contacting the nucleic acids with a fluorescent cyanine dye and monitoring the change in fluorescence emission of the dye.</p> |
