摘要 |
There is disclosed the use of O- beta -D-galactopyranosyl-D-xyloses, particularly 2-O- beta -D-galactopyranosyl-D-xylose and 3-O- beta -D-galactopyranosyl-D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. There is also disclosed a process for producing O- beta -D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of beta -D-galactopyranosyl in the presence of an enzyme beta -galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37 DEG C; deactivating the beta -galactosidase by heating at 100 DEG C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/alcohol.
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申请人 |
CONSEJO SUPERIOR INVESTIGACTIONES CIENTIFICAS;UNIVERSIDAD AUTONOMA;ARAGON REYES, JUAN JOSE;CANADA VICINAY, FRANCISCO JAVIER;FERNANDEZ-MAYORALAS ALVAREZ, ALFONSO;LOPEZ ALVAREZ, ROSA;MARTIN LOMAS, MANUEL;VILLANUEVA TORREGROZA, DANIEL |
发明人 |
ARAGON REYES, JUAN JOSE;CANADA VICINAY, FRANCISCO JAVIER;FERNANDEZ-MAYORALAS ALVAREZ, ALFONSO;LOPEZ ALVAREZ, ROSA;MARTIN LOMAS, MANUEL;VILLANUEVA TORREGROZA, DANIEL |