ELECTROCHROMATOGRAPHY APPARATUS

摘要

The invention provides fittings (10, 12) for the ends of electrochromatography columns. Each fitting includes an annular electrode (42) surrounding one end of the passage electrical fields to be applied. The fitting also contains a passageway (24) for eluent flowing into or out of the column, an annular chamber for the electrode and a membrane (60) separating the passageway from the annular electrode chamber. The membrane (60) prevents gases and electrolytic products from entering the column eluent going through the chromatographic column. The membrane also isolates the electrodes from the compounds such as proteins and macromolecules being separated. Porous bed support (66) is deposited on top of the membrane (60). A buffer solution was pumped through the electrode chamber to remove the gases and electrode products. The buffer flow through the electrode chamber maintains a constant pH and conductivity so that the electrical field applied by each electrode is constant. The buffer flow also serves to remove the heat generated in the electrode chamber. The invention also provides an electrochromatrography apparatus employing these fittings.