| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new DNA, having a specific base sequence and capable of coding a new thiol protease derived from a germinated soybean cotyledon and mass-producing an enzyme capable of efficiently hydrolyzing a soybean protein with a high efficiency according to a gene recombination method. SOLUTION: This new DNA has a base sequence represented by formula I or II and is capable of coding a new thiol protease derived from a germinated soybean cotyledon and directly mass-producing the new thiol protease capable of efficiently hydrolyzing a soybean protein into amino acids or low-molecular peptides with a high efficiency according to a gene recombination method. The new DNA is obtained by extracting an RNA from the soybean cotyledon on the 10th day after seeding according to a conventional method, then separating an mRNA from the RNA, carrying out the reverse transcription polymerase chain reaction(RT-PCR) with a primer comprising a synthetic oligonucleotide, performing the reverse transcription, amplification and cloning. |
