| 摘要 |
An apparatus for quick freezing tissue specimens includes a base supporting a linear motion platform and a rotational motion platform. Each of the platforms includes a plurality of discs for covering and receiving respectfully specimen to be frozen. Each of the discs includes a chambers positioned to very quickly and rapidly disperse cryogenic fluid therein and convey away evaporated gases therefrom. The discs are flow connect to a cryogenic fluid source such as a liquid nitrogen tank and a vacuum is drawn on the gaseous side system by a vacuum pump. The discharge of the vacuum pump is returned to the cryogenic tank such that the vacuum pump both draws the cryogenic fluid through the system and pressurizes the tank so as to motivate the fluid through the system. A rotational column with a cryogenic junction conveys the cryogenic fluid to and from the platform that rotates. The junction is constructed of axially connected and rotatable quartz tubing. A plurality of channels encircle each disc and are connected to a vacuum system. A latch and trigger mechanism allows the rotatable platform to swing about an axis in an angular motion and thereafter stop, thereafter the linear platform moves linearly toward the rotational platform. The linear platform is reset by rotation of the rotational platform.
|
