| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new galactanase virtually incapable of producing monosaccharides (e.g. galactose) even after hydrolyzing dietary fibers and capable of producing oligosaccharides (e.g. galactooligosaccharide) in high yield, and to obtain a new microorganism capable of producing the above galactanase. SOLUTION: This new galactanase, galactanase S-39, has the following enzymatic characteristics: (a) action: acting on soybean fibers to produce galactooligosaccharide but nearly not isolating monosaccharides (e.g. galactose); (b) substrate specificity: acting on β-1,4 galactan but not acting on β-1,3 galactan; (c) acting pH: 3-12; and (d) acting temperature: 20-65 deg.C. The new microorganism, Bacillus sp. S-39 (FERM P-11230), is capable of producing the above new galactanase and has the following bacterial characteristics; (a) physiological characteristics etc.: (1) negative in denitrification; (2) positive in gas generation from glucose; and (3) viable pH range: >=pH6.8; and (b) negative in anaerobic growability. |
