发明名称 SEQUENCE MODIFICATION OF OLIGONUCLEOTIDE PRIMERS TO MANIPULATE NON-TEMPLATED NUCLEOTIDE ADDITION
摘要 The present invention is directed to methods for resisting or promoting template independent nucleotide addition to the 3' terminus of a DNA duplex. The process comprises amplifying a target nucleic acid using primers which comprise a 5' terminal sequence which resists or promotes non-templated nucleotide addition to the 3' terminus of the complementary nucleic acid strand. The invention is also directed to a kit for cloning 3' nucleotidylated duplex DNA.
申请公布号 CA2235327(A1) 申请公布日期 1997.05.09
申请号 CA19962235327 申请日期 1996.10.15
申请人 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, REPRESENTED BY THE SECRE TARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES;THE GOVERNMENT OF THE UNITED STATES OF AMERICA, REPRESENTED BY THE SECRE TARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES 发明人 BROWNSTEIN, MICHAEL J.;CARPTEN, JOHN D.;SMITH, JEFFREY R.
分类号 C12N15/10;C12Q1/68;(IPC1-7):C12P19/34 主分类号 C12N15/10
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