| 摘要 |
The present invention relates to using recombinant DNA technology to introduce at least one gene encoding a protein molecule into a donor pathogenic bacterium which can attach itself and invade a given eucaryotic cell. Following invasion of the eucaryotic cell, the donor pathogenic bacterium then expresses the gene within itself and then exports the protein into the recipient eucaryotic cell resulting in the transfer of the expressed protein from the donor pathogenic bacterium to the eucaryotic cell. The effect, if any, the presence of this protein has on the recipient eucaryotic cell is then determined. In an alternate embodiment of the invention, the donor pathogenic bacterium is attenuated or avirulent. In a further embodiment, the gene expressed in the donor pathogenic bacterium is heterologous to the genome of the recipient eucaryotic cell and the genome of the donor pathogenic bacterium. In yet a further embodiment, the gene expressed in the donor pathogenic bacterium is heterologous to the genome of the recipient eucaryotic cell and is homologous to the genome of the donor pathogenic bacterium. In yet a further embodiment, the gene expressed in the donor pathogenic bacterium is homologous to the genome of the recipient eucaryotic cell and is heterologous to the genome of the donor pathogenic bacterium. |
