Transgenic protein production

摘要

DNA hybrid construct for efficient targeting expression of a protein in the mammary glands of a transgenic animal, with limited ectopic expression in other tissues. A transgenic mammal is disclosed which has a beta -lactoglobulin (BLG)/human serum albumin (HSA) cDNA hybrid construct incorporated in its genome. The BLG construct is comprised of BLG 5'-flanking sequences (e.g. promoter) and BLG intragenic sequences in conjunction with a desired cDNA such of HSA, or a minigene (coding sequences and less than all intron sequences) or a gene (coding sequences and all intron sequences) assembled for the purpose of targeting expression of the product of the encoding "DNA". <IMAGE>