PROCESS FOR THE PREPARATION OF A SINGLE CHAIN FV ANTIBODY FRAGMENT

摘要

A process for producing monomeric signal chain Fv antibody molecules in insect host cells is disclosed which comprises transforming insect host cells with an expression vector comprising a DNA sequence encoding a single chain Fv antibody polypeptide and a signal peptide, under the control of which the single chain Fv antibody molecule is secreted, and subjecting the secreted single chain Fv antibody molecules to a size separation step in order to isolate monomeric single chain Fv antibody molecules from oligomers thereof.