发明名称 |
ANTI ALPHA-GALACTOSYL SCREENING TECHNIQUE |
摘要 |
Compounds and libraries are labeled with a galactosyl epitope and then screened in accordance with an assay involving cells having a characteristic of interest. Conveniently, the screening may embody target cells, where the compounds are brought in contact with the cells. Each of the compounds carries with it the information of its identity or method of synthesis. After washing away non-specifically bound compounds, blood may be applied to the cells, whereby antibody binding to the galactosyl epitope initiates the complement cascade. Plaques are identified and the compound associated with the plaque identified. The formation of the plaque demonstrates that the compound has specific affinity for the target cell, binding of the compound to the cell does not interfere with binding of the antibody, and that the complex is capable of cytotoxic activity by means of the complement cascade. |
申请公布号 |
WO9715831(A1) |
申请公布日期 |
1997.05.01 |
申请号 |
WO1996US15448 |
申请日期 |
1996.09.27 |
申请人 |
SANGSTAT MEDICAL CORPORATION;LUSSOW, ALEXANDER, R.;BUELOW, ROLAND;POULETTY, PHILIPPE |
发明人 |
LUSSOW, ALEXANDER, R.;BUELOW, ROLAND;POULETTY, PHILIPPE |
分类号 |
G01N33/53;C07K1/04;C07K9/00;C12Q1/02;G01N33/15;G01N33/50;G01N33/566;G01N33/569;G01N33/66 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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