METHOD FOR DETERMINING THE PRESENCE OF DIABETES ASSOCIATED ANTIBODIES OR ISLET CELL DIRECTED ANTIBODIES

摘要

The present disclosure relates to the application of genetic engineering to provide artificial beta cells, i.e. cells which can secrete insulin in response to glucose. This is achieved preferably through the introduction of one or more genes selected from the insulin gene, glucokinase gene, and glucose transporter gene, so as to provide an engineered cell having all three of these genes in a biologically functional and responsive configuration. Assays for detecting the presence of diabetes-associated antibodies in biological samples using these and other engineered cells expressing diabetes-associated epitopes are described. Also disclosed are methods for the large-scale production of insulin by perfusing artificial beta cells, grown in liquid culture, with glucose-containing buffers.