发明名称 |
Method for identifying cells committed to apoptosis by determining cellular phosphotyrosine content |
摘要 |
A novel approach to study changes in protein tyrosine phosphorylation during apoptosis, and thereby identify cells committed to apoptosis is presented, methods used to study apoptosis and tyrosine phosphorylation at the single cell level are combined to study directly whether apoptosis in hematopoietic cells is associated with changes in cellular phosphotyrosine levels. The changes in cellular phosphotyrosine content strongly correlated with the appearance of features of cell death such as cell shrinkage, DNA fragmentation and loss of membrane integrity.
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申请公布号 |
US5624808(A) |
申请公布日期 |
1997.04.29 |
申请号 |
US19950412256 |
申请日期 |
1995.03.28 |
申请人 |
BECTON DICKINSON AND COMPANY |
发明人 |
THOMPSON, PETER;LUND-JOHANNSEN, FRIDJTOF |
分类号 |
G01N33/569;G01N33/68;(IPC1-7):G01N33/53;G01N33/536;G01N33/574 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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