发明名称 |
Improved enzymatic coupling reaction of N-protected-L-aspartic acid and phenylalanine methyl ester |
摘要 |
The invention relates to a process for the preparation of N-protected-L-aspartyl-L-phenylalanine methyl ester by enzymatic coupling of an N-protected-L-aspartic acid and L- or DL-phenylalanine methyl ester in an aqueous solution with formation of a precipitate using a thermolysin-like protease enzyme, wherein a water-immiscible organic solvent is added to and blended with the reaction system during the formation of the precipitate in the course of the coupling reaction, which organic solvent has a relatively high affinity for the precipitate. The process is particularly suitable as a continuous process. The method results in lower enzyme deactivation during and after the enzymatic coupling reaction and in larger and thicker precipitated crystals. |
申请公布号 |
EP0768384(A1) |
申请公布日期 |
1997.04.16 |
申请号 |
EP19960202653 |
申请日期 |
1996.09.23 |
申请人 |
HOLLAND SWEETENER COMPANY V.O.F. |
发明人 |
HARADA, TSUNEO;IRINO, SHIGEAKI;KUNISAWA, YUKIO;OYAMA, KIYOTAKA |
分类号 |
C07K5/072;C12P21/02 |
主分类号 |
C07K5/072 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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