发明名称 |
VERFAHREN ZUM NACHWEIS VON MUTATIONEN |
摘要 |
<p>A method for detecting mutations, such as a single base change or an addition or deletion of about one to four base pairs, is based on the use of a DNA mismatch-binding protein, such as MutS, which binds to a nucleic acid hybrid having a single base mismatch or unpaired base or bases, thereby allowing the detection of mutations involving as little as one base change in a nucleotide sequence. Such a method is useful for diagnosing a variety of important disease states or susceptibilities, for example, detecting the presence of a mutated oncogen. The method shares the efficiency advantages of PCR-based methods but is much simpler and cheaper to perform. Also provided is a kit useful for practicing the method of the present invention.</p> |
申请公布号 |
AT151114(T) |
申请公布日期 |
1997.04.15 |
申请号 |
AT19920917193T |
申请日期 |
1992.07.17 |
申请人 |
UPSTATE BIOTECHNOLOGY, INC. |
发明人 |
WAGNER, ROBERT, E., JR.;RADMAN, MIROSLAV |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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