A neural network assisted multi-spectral segmentation system

摘要

A neural network assisted multi-spectral segmentation method and system. According to the invention, three images having different optical bands are acquired for the same micrographic scene of a biological sample. The images are processed and a cellular material map is generated identifying cellular material. The cellular material map is then applied to a neural network. The neural network classifies the cellular material map into nuclear objects and cytoplasmic objects by determining a threshold surface in the 3-dimensional space separating the cytoplasmic and nuclear regions. In another aspect, the neural network comprises a hardware-encoded algorithm in the form of a look-up table.